Factors That Affect Enzyme Reaction Rate

Lab Report F bendors That Affect Enzymes answer Rate Name of lab Effects of temperature, pH, Enzyme Concentration, and Substrate Concentration on Enzymatic Activity Introduction Enzymes are the most important types of proteins, they execute as catalysis (speed up chemical reactions). If enzymes didnt exist, biochemical reactions would act to slowly and they couldnt keep up with the metabolic functions. Enzymes have a three-dimensional structure that is really complex. This structure consists of one or more(prenominal) polypeptide chains, they form an active site, which is an area in which the substrate eventually forget fit.The four factors that affect the act and reaction rate of an enzyme are temperature, pH, enzyme compactness and substrate concentration. Research Question/Hypothesis The hypothesis is that when the temperature is higher than 40? C, the enzyme catalysis will increase. That the enzyme activity with the pH effect is that if it isnt at the optimum pH value th ere will be a total loss of the enzyme activity. As the change in enzyme concentration increases the activity rate will increase if there is a proportional tot up of enzyme concentration and substrate.Variables Independent pH, enzyme concentration, substrate concentration and enzymatic activity. Dependent the reaction rate Control variable temperature and amount of substrates and enzymes added. Materials Phosphate Buffers Beaker Catechol Potato succus Parafilm Test Tubes Procedure To story the effect of temperature 1. Three different tally provides where filled with 3mL of inorganic inorganic phosphate. 2. They were set in three different temperature settings. First resistance was placed in an ice-water bath for ten minutes, the second in a room temperature until 21?C was r for distributively oneed, and the third supply was placed in a beaker of warm water. To study the effect of pH 1. The pH determine of pH 4, pH 6, pH 7, pH 8, and pH 10 was use to fill five separate ass ay thermionic vacuum tobacco pipes with 3 mL each. 2. To each test tube, ten force outs of catechol and potato juice were added, each tube was cover with Parafilm, and upside-down several times to potpourri the contents. After letting each tube stand for three to five minutes the tubes were inverted at one minute intervals. To study the effect of enzyme concentration 1.The steps complicate four test tubes that were tagged A, B, C, and D. 2. The contents added to tube A are 3mL positivistic 20 drops of pH 7 phosphate cushion, but no potato juice. To tube B, 3 mL confident(p) 15 drops of pH 7 phosphate buffer and 15 drops of potato juice. The amount of pH 7 phosphate buffer added to test tube C are 3 mL plus 10 drops of potato juice. To test tube D, 3 mL of pH 7 phosphate buffer and 20 drops of potato juice were added. 3. Then, each tube was cover in Parafilm and inverted several times to mix the contents.After doing so, 10 drops of catechol were added to each tube and covere d with Parafilm and inverted several times to mix the contents. 4. After allowing each tube to stand for three to four minutes the tubes were mixed at one-minute intervals. To study the effects of substrate concentration 1. Eight test tubes that were involved. 2. These test tubes were labeled 1, 2, 4, 8, 16, 24, 32, and 48. Next, each test tube had a certain amount of phosphate buffer and catechol was added to each. 3.To test tube 1 5 mL plus 47 drops of pH 7 phosphate buffer and 1 drop of catechol test tube 2 5 mL plus 46 drops of pH 7 phosphate buffer and 3 drops of catechol test tube 4 5 mL plus 44 drops of pH 7 phosphate buffer and 4 drops of catechol test tube 8 5 mL plus 40 drops of pH 7 phosphate buffer and 8 drops of catechol test tube 16 5 mL plus 32 drops of pH 7 phosphate buffer and 16 drops of catechol test tube 24 5 mL plus 24 drops of pH 7 phosphate buffer and 24 drops of catechol test tube 32 5 Ml plus 16 drops of pH 7 phosphate buffer and 32 drops of catechol and te st tube 48 5 mL of pH 7 phosphate buffer and 48 drops of catechol. 4. Then, each tube was covered with Parafilm and inverted several times to mix the contents. Also, to each of the tubes, 30 drops of potato juice were added and covered with Parafilm and inverted several times to mix the contents. The test tubes set at room temperature for five minutes and were mixed at one minute intervals.